Information
Drawing
Staining
Knowledge
marked
unmarked

Specimen Nr. 01C

Specimen:

Rat fetus

Staining:

Hopa

Magnification:

40x

Important structures :

1.Meckel cartilage
2.Area of osteoprogenitor cells
3.Osteoblasts
4.Osteoclasts
5.Bone trabeculae
6.Lymphatic vessel (lumen)
7.Blood vessel (lumen)
Die intensiv blau gefärbten Knochenbälkchen des Unterkiefers der Ratte sind durch desmale Ossifikation entstanden. Eingemauert in den Knochenbälkchen sind die Osteozyten. In der Nähe der Knochenbälkchen befinden sich Areale dicht zusammenliegender Knochenvorläuferzellen (Osteoprogenitorzellen). Direkt den Knochenbälkchen sitzen die Osteoblasten auf. Auch Osteoklasten, Zellen die durch ihre Größe auffallen, kommen vor. Beachte die zahlreichen Blut- und Lymphgefäße (letztere ohne Erythrozyten).

Legende:

Meckel cartilage
Area of osteoprogenitor cells
Osteoblasts
Osteoclasts
Bone trabeculae
Lymphatic vessel (lumen)
Blood vessel (lumen)

HOPA

Structures Colour
Nuclei brown
Cytoplasm grey-blue
Connective-tissue fibres
- Collagen
- Reticular
- Elastic
blue
blue
-
Myocytes grey-blue to grey-green
Erythrocytes yellow-orange

Dyes:

HOPA = Hemalaun, Orange G, Phosphormolybdenic acid, Aniline blue

Cell nuclei are stained with hemalaun or Weigerts iron-hematoxylin and then counterstained with a dye mixture of orange-phosphormolybden with aniline blue.

Der Meckel-Knorpel ist ein embryonaler Knorpel des 1. Kiemenbogens. Er bildet das transitorische Skelett der Mundbodenregion, auf das sich der Unterkiefer (Mandibula) als Deck- oder Bindegewebsknochen (=Knochen, der durch desmale Ossifikation entstanden ist) auflegt.
Home
Tutor
Help
Exit
Boxes

Magnification:

2x

Magnification:

16x

Magnification:

40x

Magnification:

100x

Magnification:

250x

Magnifications
Meckel cartilage
Area of osteoprogenitor cells
Osteoblasts
Osteoblasts
Osteoclasts
Osteoclasts
Bone trabeculae
Lymphatic vessel (lumen)
Blood vessel (lumen)

HistoNet2000 - Help

1. Organization of the screen surface

Right side: histologic specimen
Left side: information about the specimen (above) and general program functions (below)

2.Histologic specimen

Pull the mouse across the histologic specimen for training purposes. A small square with exclamation marks (dynamic labels) will appear where there is an important structure. You should then decide what structure this could be. To check your result, simply click the appropriate square, and the correct label will appear. The option “marked” allows you to see all labels for all structures simultaneously. These can be removed by clicking “unmarked”. This reactivates the dynamic labels.

3. Complementary information

Info: general information about the specimen, as well as a list of the dynamic labels
Drawing: schematic drawing of the specimen
Staining: information about the staining method for this specimen
Knowledge: short texts with basic histologic information, presently deactivated

4. General Program Functions

Home: returns you to the “start” page
Tutor: how to contact the HistoNet Team
Help: Instructions for Use appear
Exit: closes down the HistoNet program
Boxes: goes back to the other specimen of a topic
VM: provides virtual microscopy

We hope you will enjoy working with HistoNet2000 and learn a lot from it!

Cose help