Specimen Nr. 03D

Specimen:

Duct of epididymis (Sheep)

Staining:

Hopa

Magnification:

160x

Important structures :

1.	Pseudostratified columnar epithelium showing stereocilia
2.	Stereocilia
3.	Basal epithelial cells that do not reach the surface
4.	Spermatocytes

Gut zu erkennen ist das mehrreihig hochprismatische Epithel, welches apikal untereinander ?verklebte? Stereozilien trägt und an dessen basalem Ende wir die kleinen Ersatzzellen mit ihren rundlichen Kernen entdecken. Im Lumen finden wir unbewegliche Spermatozoen. Umrandet wird die Epithelschicht von einem schmalen Saum glatter Muskulatur.

Legende:

	Pseudostratified columnar epithelium showing stereocilia
	Stereocilia
	Basal epithelial cells that do not reach the surface
	Spermatocytes

HOPA

Structures	Colour
Nuclei	brown
Cytoplasm	grey-blue
Connective-tissue fibres - Collagen - Reticular - Elastic	blue blue -
Myocytes	grey-blue to grey-green
Erythrocytes	yellow-orange

Dyes:

HOPA = Hemalaun, Orange G, Phosphormolybdenic acid, Aniline blue

Cell nuclei are stained with hemalaun or Weigerts iron-hematoxylin and then counterstained with a dye mixture of orange-phosphormolybden with aniline blue.

Spermatozoen werden in den Tubuli seminiferi, den Hodenkanälchen, gebildet. Von dort werden sie über das Rete testis und den Ductus epididymidis (Nebenhoden) in den Ductus deferens (Samenleiter) transportiert. Erst dort erlangen sie Beweglichkeit.

Magnification:

64x

Magnification:

160x

Pseudostratified columnar epithelium showing stereocilia

Basal epithelial cells that do not reach the surface

HistoNet2000 - Help

1. Organization of the screen surface

Right side: histologic specimen
Left side: information about the specimen (above) and general program functions (below)

2.Histologic specimen

Pull the mouse across the histologic specimen for training purposes. A small square with exclamation marks (dynamic labels) will appear where there is an important structure. You should then decide what structure this could be. To check your result, simply click the appropriate square, and the correct label will appear. The option “marked” allows you to see all labels for all structures simultaneously. These can be removed by clicking “unmarked”. This reactivates the dynamic labels.

3. Complementary information

Info: general information about the specimen, as well as a list of the dynamic labels
Drawing: schematic drawing of the specimen
Staining: information about the staining method for this specimen
Knowledge: short texts with basic histologic information, presently deactivated

4. General Program Functions

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