Specimen Nr. 02B

Specimen:

Gelatinous connective tissue, Umbilical cord (Human being)

Staining:

Azan

Magnification:

64x

Important structures :

1.	Cells and extracellular matrix of gelatinous connective tissue
2.	Amniotic epithel
3.	Fixation artifact
4.	Mesenchymal cell

Dieser Ausschnitt der Nabelschnur läßt den Aufbau des gallertigen Bindegewebes erkennen. Zwischen den spindelförmigen, rot angefärbten Zellen liegt die extrazelluläre Matrix, die hier blau angefärbt ist. Das einschichtige isoprismatische Epithel stellt das Amnionepithel dar. Der hier zu sehende breite Spalt zwischen Amnionepithel und Gallertgewebe ist ein durch Fixierung bedingter Schrumpfungsartefakt, der im Nativzustand nicht vorkommt.

Legende:

	Cells and extracellular matrix of gelatinous connective tissue
	Amniotic epithel
	Fixation artifact
	Mesenchymal cell

Gelatinous connective tissue (Umbilical cord)[bu]

1.	Mesenchymal cells
2.	Small collagen fibrils
3.	Gelatinous intercellular substance

Azan (Heidenhain)

Azan dye is a multiple or trichromatic staining method. It differentiates extracellular connective-tissue fibres, tissues, and cell components.

Structures	Colour
Nuclei	red
Cytoplasm	reddish
Connective-tissue fibres - Collagenic - Reticular - Elastic	blue blue unstained or reddish
Muscle tissue	red-orange

Dyes:

Azokarmin	Azokarmin is a red dye used mainly to stain cell nuclei.
Aniline blue- orange G	This dye is used for counterstaining.

Das gallertige Bindegewebe ist die zweite Art des embryonalen Bindegewebes. Im Gegensatz zum pluripotenten Mesenchym kann sich das gallertige Bindegewebe jedoch nicht mehr weiter differenzieren. Es enthält Zellen, die neben einer lichtmikroskopisch ungeformten, hyaluronsäurehaltigen Grundsubstanz fibrilläre Proteine synthetisieren.

Magnification:

64x

Magnification:

128x

Cells and extracellular matrix of gelatinous connective tissue

HistoNet2000 - Help

1. Organization of the screen surface

Right side: histologic specimen
Left side: information about the specimen (above) and general program functions (below)

2.Histologic specimen

Pull the mouse across the histologic specimen for training purposes. A small square with exclamation marks (dynamic labels) will appear where there is an important structure. You should then decide what structure this could be. To check your result, simply click the appropriate square, and the correct label will appear. The option “marked” allows you to see all labels for all structures simultaneously. These can be removed by clicking “unmarked”. This reactivates the dynamic labels.

3. Complementary information

Info: general information about the specimen, as well as a list of the dynamic labels
Drawing: schematic drawing of the specimen
Staining: information about the staining method for this specimen
Knowledge: short texts with basic histologic information, presently deactivated

4. General Program Functions

Home: returns you to the “start” page
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Help: Instructions for Use appear
Exit: closes down the HistoNet program
Boxes: goes back to the other specimen of a topic
VM: provides virtual microscopy

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