Die extrazelluläre Matrix des gallertigen Bindegewebes der Nabelschnur enthält kollagene Fibrillen. Diese sind teils locker angeordnet, teils miteinander verfilzt (intensiver blau gefärbte Areale). In die Matrix eingestreut sind die abgeplatteten, rot gefärbten Zellen, die einen weitmaschigen Zellverband bilden.

Legende:

	Cell of gelatinous connective tissue
	Extracellular matrix with collagen fibrils

Azan (Heidenhain)

Azan dye is a multiple or trichromatic staining method. It differentiates extracellular connective-tissue fibres, tissues, and cell components.

Structures	Colour
Nuclei	red
Cytoplasm	reddish
Connective-tissue fibres - Collagenic - Reticular - Elastic	blue blue unstained or reddish
Muscle tissue	red-orange

Dyes:

Azokarmin	Azokarmin is a red dye used mainly to stain cell nuclei.
Aniline blue- orange G	This dye is used for counterstaining.

Anders als beim Mesenchym sind beim gallertigen Bindegewebe bereits geformte Bestandteile, die kollagenen Fibrillen, in der extrazellulären Matrix vorhanden. Als lichtmikroskopisch nicht erkennbare Strukturen enthält die Matrix Glykosaminoglykane, vor allem die stark wasserbindende Hyaluronsäure. Dieser Aufbau verleiht der Nabelschnur Zugfestigkeit und einen hohen Turgor und schützt sie so trotz ihres spiraligen Verlaufs vor dem Abknicken.

HistoNet2000 - Help

1. Organization of the screen surface

Right side: histologic specimen
Left side: information about the specimen (above) and general program functions (below)

2.Histologic specimen

Pull the mouse across the histologic specimen for training purposes. A small square with exclamation marks (dynamic labels) will appear where there is an important structure. You should then decide what structure this could be. To check your result, simply click the appropriate square, and the correct label will appear. The option “marked” allows you to see all labels for all structures simultaneously. These can be removed by clicking “unmarked”. This reactivates the dynamic labels.

3. Complementary information

Info: general information about the specimen, as well as a list of the dynamic labels
Drawing: schematic drawing of the specimen
Staining: information about the staining method for this specimen
Knowledge: short texts with basic histologic information, presently deactivated

4. General Program Functions

Home: returns you to the “start” page
Tutor: how to contact the HistoNet Team
Help: Instructions for Use appear
Exit: closes down the HistoNet program
Boxes: goes back to the other specimen of a topic
VM: provides virtual microscopy

We hope you will enjoy working with HistoNet2000 and learn a lot from it!

Specimen Nr. 02C

Specimen:

Staining:

Magnification:

Important structures :

Legende:

Azan (Heidenhain)

Dyes:

HistoNet2000 - Help