Information
Drawing
Staining
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Specimen Nr. 03B

Specimen:

Cerebrum (Human being)

Staining:

Nissl stain

Magnification:

80x

Important structures :

1.Cortex, layer IV
2.Cortex, layer V: groups of pyramidal cells
Ausschnitt aus dem Cortex. Man sieht zahlreiche größere und kleinere neuronale Perikarya, deren Fortsätze nicht zu erkennen sind. Das Neuropil erscheint in einer Nissl-Färbung völlig unstrukturiert. Etwa in der Bildmitte muß man sich horizontal verlaufend die Grenze zwischen Schicht IV und V vorstellen. Die typischen dreiecksförmigen Perikaryen der Pyramidenzellen (mit gelegentlich zu erkennendem Stamm des apikalen Dendriten) beschränken sich weitgehend auf die untere Bildhälfte.

Legende:

Cortex, layer IV
Cortex, layer V: groups of pyramidal cells

Nissl stain

Dyes: Cresyl violet, Toluidine blue, or Thionin:

These dyes bind to basophilic structures, like RNA and DNA. They especially stain Nissl substance and nucleoli blue or violet.

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Tutor
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Boxes

Magnification:

2x

Magnification:

80x

Magnification:

200x

Magnifications
Cortex, layer IV
Cortex, layer V: groups of pyramidal cells
Cortex, layer V: groups of pyramidal cells
Cortex, layer V: groups of pyramidal cells

HistoNet2000 - Help

1. Organization of the screen surface

Right side: histologic specimen
Left side: information about the specimen (above) and general program functions (below)

2.Histologic specimen

Pull the mouse across the histologic specimen for training purposes. A small square with exclamation marks (dynamic labels) will appear where there is an important structure. You should then decide what structure this could be. To check your result, simply click the appropriate square, and the correct label will appear. The option “marked” allows you to see all labels for all structures simultaneously. These can be removed by clicking “unmarked”. This reactivates the dynamic labels.

3. Complementary information

Info: general information about the specimen, as well as a list of the dynamic labels
Drawing: schematic drawing of the specimen
Staining: information about the staining method for this specimen
Knowledge: short texts with basic histologic information, presently deactivated

4. General Program Functions

Home: returns you to the “start” page
Tutor: how to contact the HistoNet Team
Help: Instructions for Use appear
Exit: closes down the HistoNet program
Boxes: goes back to the other specimen of a topic
VM: provides virtual microscopy

We hope you will enjoy working with HistoNet2000 and learn a lot from it!

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