Specimen Nr. 03C

Specimen:

Cerebrum (Human being)

Staining:

Nissl stain

Magnification:

200x

Important structures :

1.	Pyramidal cell: perikaryon with nucleus, nucleolus, and beginning of apical dendrite
2.	Perikaryon of an interneuron
3.	2 glial cell nuclei (probably astrocytes)
4.	Capillary

Ausschnitt aus der Schicht V des Cortex mit neuronalen und glialen Zellkörpern. Am oberen Bildrand finden sich zwei Perikarya von Pyramidenzellen, deren apikale Dendriten etwas von der vertikalen Ausrichtung abweichen. Die Unterscheidung von kleinen Interneuronen und Gliazellen ist nicht immer einfach. Von der rechten oberen Bildecke erstreckt sich nach links unten im Bogen eine längsgeschnittene Kapillare. Die hier sichtbaren Zellkerne gehören entweder zu Endothelzellen oder zu Perizyten. Das Flechtwerk der Fortsätze im Neuropil ist bei dieser Färbung nicht zu erkennen.

Legende:

	Pyramidal cell: perikaryon with nucleus, nucleolus, and beginning of apical dendrite
	Perikaryon of an interneuron
	2 glial cell nuclei (probably astrocytes)
	Capillary

Nissl stain

Dyes: Cresyl violet, Toluidine blue, or Thionin:

These dyes bind to basophilic structures, like RNA and DNA. They especially stain Nissl substance and nucleoli blue or violet.

Die Pyramidenzellen sind die Output-Zellen des Cortex. Ihre Axone ziehen entweder zu Rindenfeldern in der gleichen Hemisphäre (Assoziationsfasern), zu Rindenfeldern in der kontralateralen Hemisphäre (Kommissurenfasern) oder zu subcorticalen Strukturen einschließlich Hirnstamm und Rückenmark (Projektionsfasern). Neurotransmitter: Glutamat

Magnification:

80x

Magnification:

200x

Pyramidal cell: perikaryon with nucleus, nucleolus, and beginning of apical dendrite

Perikaryon of an interneuron

2 glial cell nuclei (probably astrocytes)

HistoNet2000 - Help

1. Organization of the screen surface

Right side: histologic specimen
Left side: information about the specimen (above) and general program functions (below)

2.Histologic specimen

Pull the mouse across the histologic specimen for training purposes. A small square with exclamation marks (dynamic labels) will appear where there is an important structure. You should then decide what structure this could be. To check your result, simply click the appropriate square, and the correct label will appear. The option “marked” allows you to see all labels for all structures simultaneously. These can be removed by clicking “unmarked”. This reactivates the dynamic labels.

3. Complementary information

Info: general information about the specimen, as well as a list of the dynamic labels
Drawing: schematic drawing of the specimen
Staining: information about the staining method for this specimen
Knowledge: short texts with basic histologic information, presently deactivated

4. General Program Functions

Home: returns you to the “start” page
Tutor: how to contact the HistoNet Team
Help: Instructions for Use appear
Exit: closes down the HistoNet program
Boxes: goes back to the other specimen of a topic
VM: provides virtual microscopy

We hope you will enjoy working with HistoNet2000 and learn a lot from it!