Specimen Nr. 05A


Cerebellum (Human being)





Important structures :

1.White matter, medulla
2.Grey matter, cortex, granular layer
3.Grey matter, cortex, molecular layer
4.Sulcus (subarachnoid space)
Das Präparat zeigt zwei quergeschnittene Windungen der Kleinhirnrinde, die durch entsprechende Furchen getrennt sind. In diese zieht der Subarachnoidalraum hinein. Die Pia mater ist teilweise vom darunterliegenden zentralnervösen Gewebe abgelöst.


White matter, medulla
Grey matter, cortex, granular layer
Grey matter, cortex, molecular layer
Sulcus (subarachnoid space)

Purkinje cell of cerebrum[bu]

1. Dendrites
2. Cell body
3. Axon

Myelin-sheath stains

Weigert iron-hematoxylin stain

Structures Colour
Nuclei black to blue-black
Cytoplasm -
Connective-tissue fibres
    - Collagen
    - Reticular
    - Elastic

black to blue-black
Myocytes black to blue-black
Erythrocytes black to blue-black


Iron-hematoxylin: is a hematoxylin and stains far more intensively than hemalaun. To be able to stain myelin sheaths with Weigert dye, the lipids in the myelin must be preserved while preparing the specimen. The myelin sheaths turn black to blue-black.
Luxol fast blue: Only the neurokeratin remains in normal paraffin fixation of myelin sheaths. Luxol fast blue has a specific affinity to this lipoprotein and stains the myelin sheaths bright blue.
Osmium tetroxide The specimens must be prepared as frozen sections. Osmium tetroxide is deposited on the double bonds of unsaturated fatty acids and produces reaction products which make even the thinnest myelin sheaths visible through black stain.









White matter, medulla
Grey matter, cortex, granular layer
Grey matter, cortex, molecular layer
Sulcus (subarachnoid space)

HistoNet2000 - Help

1. Organization of the screen surface

Right side: histologic specimen
Left side: information about the specimen (above) and general program functions (below)

2.Histologic specimen

Pull the mouse across the histologic specimen for training purposes. A small square with exclamation marks (dynamic labels) will appear where there is an important structure. You should then decide what structure this could be. To check your result, simply click the appropriate square, and the correct label will appear. The option “marked” allows you to see all labels for all structures simultaneously. These can be removed by clicking “unmarked”. This reactivates the dynamic labels.

3. Complementary information

Info: general information about the specimen, as well as a list of the dynamic labels
Drawing: schematic drawing of the specimen
Staining: information about the staining method for this specimen
Knowledge: short texts with basic histologic information, presently deactivated

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