Specimen Nr. 01A

Specimen:

Vocal fold (Human being)

Staining:

van Gieson

Magnification:

2,5x

Important structures :

1.Vestibular fold (false vocal cord)
2.Vocal fold (true vocal cord)
3.Seromucous glands
4.Striated muscle tissue
5.Laryngeal ventricle

Die Abbildung zeigt einen Frontalschnitt durch das mittlere Stockwerk des Kehlkopfs, Cavitas laryngis intermedia oder Glottis, das zur Hälfte zu sehen ist. Kranial wird es von der Taschenfalte (Plica vestibularis), kaudal von der Stimmfalte (Plica vocalis) begrenzt, seitlich ist der Raum zum Ventriculus laryngis erweitert.
Hinweis: Zum besseren Verständnis dieses histologischen Schnittes trägt das Schema der makroskopischen Anatomie des Kehlkopfes bei. Klicken Sie auf die Option Zeichnung!

Legende:

Vestibular fold (false vocal cord)
Vocal fold (true vocal cord)
Seromucous glands
Striated muscle tissue
Laryngeal ventricle

Respiratory system (Overview)[hs]

1. Nasal cavity
2. Pharynx
3. Larynx
4. Trachea
5. Main bronchi
6. Lung
  1. air-conducting portions
  2. respiratory portion (gas exchange)
7. Ribs
8. Mediastinum
9. Diaphragm

Giesons trichromatic stain

This is the dye most commonly used to stain connective tissue.

Structures Colour
Nuclei black-brown
Cytoplasm yellow-brown
Connective-tissue fibres
- Collagen
- Reticular
- Elastic

red
-
yellow
Muscle tissue yellow
Erythrocytes yellow

Dyes:

Weigerts iron hematoxylin This dye is one of the hematoxylins, but stains much more intensively than hemalaun. It is used with van Gieson’s dye to stain cell nuclei.
Picro-fuchsin or Picro-thiacid mixture This dye solution simultaneously stains connective tissue red and muscle tissue yellow (simultaneous stain).
Funktionen des Kehlkopfes sind
  1. Schutzeinrichtung für die Luftwege durch Verschluß beim Schlucken und Husten
  2. Erzeugung von Lauten bei der Stimmbildung. Hierbei verschließen die zwei Stimmfalten (Plicae vocales) die Stimmritze. Durch den Luftstrom bei der Ausatmung geraten sie in Schwingung, wodurch - ähnlich wie bei einem Blasinstrument - Töne entstehen.
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Magnification:

2,5x

Magnification:

81x

Magnification:

102x

Magnifications
Vestibular fold (false vocal cord)
Vocal fold (true vocal cord)
Seromucous glands
Striated muscle tissue
Laryngeal ventricle

HistoNet2000 - Help

1. Organization of the screen surface

Right side: histologic specimen
Left side: information about the specimen (above) and general program functions (below)

2.Histologic specimen

Pull the mouse across the histologic specimen for training purposes. A small square with exclamation marks (dynamic labels) will appear where there is an important structure. You should then decide what structure this could be. To check your result, simply click the appropriate square, and the correct label will appear. The option “marked” allows you to see all labels for all structures simultaneously. These can be removed by clicking “unmarked”. This reactivates the dynamic labels.

3. Complementary information

Info: general information about the specimen, as well as a list of the dynamic labels
Drawing: schematic drawing of the specimen
Staining: information about the staining method for this specimen
Knowledge: short texts with basic histologic information, presently deactivated

4. General Program Functions

Home: returns you to the “start” page
Tutor: how to contact the HistoNet Team
Help: Instructions for Use appear
Exit: closes down the HistoNet program
Boxes: goes back to the other specimen of a topic
VM: provides virtual microscopy

We hope you will enjoy working with HistoNet2000 and learn a lot from it!

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