Specimen Nr. 01B

Specimen:

Vocal fold (Human being)

Staining:

van Gieson

Magnification:

81x

Important structures :

1.	Pseudostratified cilliated epithelium of vestibular fold (false vocal cord)
2.	Lamina propria (subepithelial connective tissue)

Das mehrreihige Flimmerepithel, das die Taschenfalte (Plica vestibularis) überzieht, ist bei stärkerer Vergrößerung zusammen mit dem darunterliegenden Bindegewebe der Lamina propria zu sehen. Das subepitheliale Bindegewebe enthält neben den ortsständigen Fibrozyten zahlreiche freie Bindegewebszellen, wie z.B. Lymphozyten. Die hier vorkommenden sero-mukösen Drüsen sind nicht abgebildet.

Legende:

	Pseudostratified cilliated epithelium of vestibular fold (false vocal cord)
	Lamina propria (subepithelial connective tissue)

Larynx[hs]

1.	Epiglottis
2.	Thyroid cartilage
3.	Cricoid cartilage
4.	Vestibular fold
5.	Vocal fold
6.	M. vocalis
7.	Seromucous gland
8.	Trachea
9.	Laryngeal ventricle

Giesons trichromatic stain

This is the dye most commonly used to stain connective tissue.

Structures	Colour
Nuclei	black-brown
Cytoplasm	yellow-brown
Connective-tissue fibres - Collagen - Reticular - Elastic	red - yellow
Muscle tissue	yellow
Erythrocytes	yellow

Dyes:

Weigerts iron hematoxylin	This dye is one of the hematoxylins, but stains much more intensively than hemalaun. It is used with van Gieson’s dye to stain cell nuclei.
Picro-fuchsin or Picro-thiacid mixture	This dye solution simultaneously stains connective tissue red and muscle tissue yellow (simultaneous stain).

Der Kehlkopf ist größtenteils von mehrreihigem Flimmerepithel ausgekleidet. Nur im Anfangsteil (Übergang Pharynx ? Larynx) und im Bereich der Stimmfalte (Plica vocalis) kommt mehrschichtiges unverhorntes Plattenepithel vor.

Magnification:

2,5x

Magnification:

81x

Magnification:

102x

Pseudostratified cilliated epithelium of vestibular fold (false vocal cord)

Lamina propria (subepithelial connective tissue)

HistoNet2000 - Help

1. Organization of the screen surface

Right side: histologic specimen
Left side: information about the specimen (above) and general program functions (below)

2.Histologic specimen

Pull the mouse across the histologic specimen for training purposes. A small square with exclamation marks (dynamic labels) will appear where there is an important structure. You should then decide what structure this could be. To check your result, simply click the appropriate square, and the correct label will appear. The option “marked” allows you to see all labels for all structures simultaneously. These can be removed by clicking “unmarked”. This reactivates the dynamic labels.

3. Complementary information

Info: general information about the specimen, as well as a list of the dynamic labels
Drawing: schematic drawing of the specimen
Staining: information about the staining method for this specimen
Knowledge: short texts with basic histologic information, presently deactivated

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