Specimen Nr. 01C

Specimen:

Vocal fold (Human being)

Staining:

van Gieson

Magnification:

102x

Important structures :

1.Nonkeratinized stratified squamous epithelium of (true) vocal cord
2.Lamina propria (subepithelial connective tissue)
3.Capillary
Ein geschichtetes unverhorntes Plattenepithel überzieht die Stimmfalte (Pilca vocalis). Im darunterliegenden Bindegewebe der Lamina propria fehlen – im Gegensatz zu dem der Taschenfalte – seromuköse Drüsen und Lymphgefäße. (Letzteres ist von Bedeutung für die Begrenzung bzw. Ausbreitung des Plattenepithel – Carcinoms der Stimmbänder.)

Legende:

Nonkeratinized stratified squamous epithelium of (true) vocal cord
Lamina propria (subepithelial connective tissue)
Capillary

Larynx[hs]

1. Epiglottis
2. Thyroid cartilage
3. Cricoid cartilage
4. Vestibular fold
5. Vocal fold
6. M. vocalis
7. Seromucous gland
8. Trachea
9. Laryngeal ventricle

Giesons trichromatic stain

This is the dye most commonly used to stain connective tissue.

Structures Colour
Nuclei black-brown
Cytoplasm yellow-brown
Connective-tissue fibres
- Collagen
- Reticular
- Elastic

red
-
yellow
Muscle tissue yellow
Erythrocytes yellow

Dyes:

Weigerts iron hematoxylin This dye is one of the hematoxylins, but stains much more intensively than hemalaun. It is used with van Gieson’s dye to stain cell nuclei.
Picro-fuchsin or Picro-thiacid mixture This dye solution simultaneously stains connective tissue red and muscle tissue yellow (simultaneous stain).

Das Kehlkopfkarzinom ist der häufigste maligne Tumor im Halsbereich. Histologisch handelt es sich dabei überwiegend um Plattenepithelkarzinome, die zu 60 % vom Stimmband ausgehen. Bei diesen sog. glottischen Karzinomen ist Heiserkeit das im Vordergrund stehende klinische Sympton.

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Magnification:

2,5x

Magnification:

81x

Magnification:

102x

Magnifications
Nonkeratinized stratified squamous epithelium of (true) vocal cord
Lamina propria (subepithelial connective tissue)
Capillary

HistoNet2000 - Help

1. Organization of the screen surface

Right side: histologic specimen
Left side: information about the specimen (above) and general program functions (below)

2.Histologic specimen

Pull the mouse across the histologic specimen for training purposes. A small square with exclamation marks (dynamic labels) will appear where there is an important structure. You should then decide what structure this could be. To check your result, simply click the appropriate square, and the correct label will appear. The option “marked” allows you to see all labels for all structures simultaneously. These can be removed by clicking “unmarked”. This reactivates the dynamic labels.

3. Complementary information

Info: general information about the specimen, as well as a list of the dynamic labels
Drawing: schematic drawing of the specimen
Staining: information about the staining method for this specimen
Knowledge: short texts with basic histologic information, presently deactivated

4. General Program Functions

Home: returns you to the “start” page
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Help: Instructions for Use appear
Exit: closes down the HistoNet program
Boxes: goes back to the other specimen of a topic
VM: provides virtual microscopy

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