Specimen Nr. 01C

Specimen:

Lymph node (Dog)

Staining:

H&E

Magnification:

100x

Important structures :

1.Adipose tissue
2.Capsule
3.Marginal sinus
4.Germinal centre of a secondary lymphoid follicle
5.Corona
6.Trabecula
7.Medullary cord of lymph node
8.Medullary sinus of lymph node
Direkt unter der Kapsel des Lymphknotens befindet sich der Randsinus (Marginalsinus), in den sich die Lymphe aus den afferenten Lymphgefäßen ergießt. Das Keimzentrum des Sekundärfollikels enthält vor allem große, sich teilende Lymphozyten und erscheint deswegen heller als die Korona, die überwiegend kleine und ruhende Lymphozyten aufweist. Im Mark liegen die Markstränge und Marksinus.

Legende:

Adipose tissue
Capsule
Marginal sinus
Germinal centre of a secondary lymphoid follicle
Corona
Trabecula
Medullary cord of lymph node
Medullary sinus of lymph node

Lymph node[wh]

1. Capsule
2. Trabecula
3. Marginal sinus
4. Afferent lymphatic vessels
5. Efferent lymphatic vessel
6. Cortex
7. Lymphatic nodule with germinal centre
8. Medullary cord of lymph node
9. Medullary sinus of lymph node

Hematoxylin and Eosin (H&E)

H&E is the most common survey stain, which shows cell nuclei and cytoplasmatic components.

Structures Colour
Nuclei blue
Cytoplasm pale red
Connective-tissue fibres
- Collagen
- Reticular
- Elastic

red
-
pale rose to red
Muscle tissue red
Erythrocytes red-orange

Dyes:

Hematoxylin (e.g. hemalaun, Weigerts or Heidenhains iron-hematoxylin)
Hematoxylin is a positively charged dye (at low pH values). It therefore colors negatively charged (basophilic) structures blue, like the DNA of nuclear chromatin, the RNA of rough endoplasmatic reticulum, and the acidic glycosaminoglycans of hyaline cartilage.
Hematoxylin used in the preparation of iron varnish is Weigerts or Heidenhains iron-hematoxylin and in the preparation of aluminum varnish is hemalaun.
Eosin Eosin is a negatively charged dye used for counterstaining. It stains all remaining structures different shades of red (acidophilic).

Thiazinrot

Thiazinrot behaves similar as eosin.
Die Kapsel wird durch mehrere afferente Lymphgefäße durchbrochen, und die Lymphe fließt in den unter der gesamten Kapsel liegenden lymphozytenarmen Spaltraum, den Randsinus (Marginalsinus). Von dort gelangt sie in den Intermediärsinus, der sich zwischen den Follikeln der Rinde befindet, darauf in das Netzwerk der Marksinus, die zu den am Hilum herausführenden efferenten Lymphgefäßen konvergieren.
Home
Tutor
Help
Exit
Boxes

Magnification:

10x

Magnification:

25x

Magnification:

100x

Magnification:

100x

Magnification:

400x

Magnifications
Adipose tissue
Capsule
Marginal sinus
Marginal sinus
Germinal centre of a secondary lymphoid follicle
Corona
Trabecula
Trabecula
Medullary cord of lymph node
Medullary cord of lymph node
Medullary sinus of lymph node

HistoNet2000 - Help

1. Organization of the screen surface

Right side: histologic specimen
Left side: information about the specimen (above) and general program functions (below)

2.Histologic specimen

Pull the mouse across the histologic specimen for training purposes. A small square with exclamation marks (dynamic labels) will appear where there is an important structure. You should then decide what structure this could be. To check your result, simply click the appropriate square, and the correct label will appear. The option “marked” allows you to see all labels for all structures simultaneously. These can be removed by clicking “unmarked”. This reactivates the dynamic labels.

3. Complementary information

Info: general information about the specimen, as well as a list of the dynamic labels
Drawing: schematic drawing of the specimen
Staining: information about the staining method for this specimen
Knowledge: short texts with basic histologic information, presently deactivated

4. General Program Functions

Home: returns you to the “start” page
Tutor: how to contact the HistoNet Team
Help: Instructions for Use appear
Exit: closes down the HistoNet program
Boxes: goes back to the other specimen of a topic
VM: provides virtual microscopy

We hope you will enjoy working with HistoNet2000 and learn a lot from it!

Cose help