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Specimen Nr. 01B

Specimen:

Tongue (Rabbit)

Staining:

Hopa

Magnification:

32x

Important structures :

1.Nonkeratinized stratified squamous epithelium with a foliate papilla
2.Connective tissue papilla
3.Taste buds
4.Furrow between two foliate papillae
5.Excretory duct
6.Lamina propria of mucosa
7.Striated muscle tissue
Jede Papilla foliata (Blattpapille) weist eine oder mehrere Epitheleinsenkungen und Bindegewebsvorwölbungen (Bindegewebspapillen) auf. Das Oberflächenepithel dieser Blattpapillen ist mehrschichtig und unverhornt. An den Seitenrändern befinden sich mehrere, dicht beieinanderliegende Geschmacksknospen. In die Gräben zwischen den Blattpapillen münden die Ausführungsgänge der serösen Spüldrüsen ein.

Legende:

Nonkeratinized stratified squamous epithelium with a foliate papilla
Connective tissue papilla
Taste buds
Furrow between two foliate papillae
Excretory duct
Lamina propria of mucosa
Striated muscle tissue

Foliate papillae[bu]

1. Taste buds
2. Excretory duct
3. Serous salivary glands (end pieces)
4. Striated muscle tissue

HOPA

Structures Colour
Nuclei brown
Cytoplasm grey-blue
Connective-tissue fibres
- Collagen
- Reticular
- Elastic
blue
blue
-
Myocytes grey-blue to grey-green
Erythrocytes yellow-orange

Dyes:

HOPA = Hemalaun, Orange G, Phosphormolybdenic acid, Aniline blue

Cell nuclei are stained with hemalaun or Weigerts iron-hematoxylin and then counterstained with a dye mixture of orange-phosphormolybden with aniline blue.

Papillen sind Erhebungen der Mundschleimhaut und des darunterliegenden Bindegewebes, die verschiedene Formen und Funktionen haben. Man unterscheidet 4 Arten:
  1. Papillae filiformes (fadenförmige Papillen)
    Sie sind sehr zahlreich und auf der gesamten Zungenoberfläche vorhanden. Sie haben mechanische Aufgaben.
  2. Papillae fungiformes (pilzförmige Papillen)
    Sie enthalten vereinzelt Geschmacksknospen und sind unregelmäßig zwischen den Papillae filiformes eingestreut.
  3. Papillae foliatae (Blattpapillen)
    Sie sind beim Menschen nur spärlich entwickelt, v.a. in der Kindheit.
  4. Papillae vallatae
    Sie befinden sich im hinteren Anteil der Zunge (V-Region) als 7-12 makroskopisch sichtbare, von einem Wall umgebene Papillen. An den Seitenwänden kommen Geschmacksknospen vor. Am Grund der Wallgräben münden die Ausführungsgänge seröser Spüldrüsen.
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Magnification:

13x

Magnification:

32x

Magnification:

81x

Magnification:

202x

Magnifications
Nonkeratinized stratified squamous epithelium with a foliate papilla
Connective tissue papilla
Taste buds
Furrow between two foliate papillae
Excretory duct
Lamina propria of mucosa
Striated muscle tissue

HistoNet2000 - Help

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Right side: histologic specimen
Left side: information about the specimen (above) and general program functions (below)

2.Histologic specimen

Pull the mouse across the histologic specimen for training purposes. A small square with exclamation marks (dynamic labels) will appear where there is an important structure. You should then decide what structure this could be. To check your result, simply click the appropriate square, and the correct label will appear. The option “marked” allows you to see all labels for all structures simultaneously. These can be removed by clicking “unmarked”. This reactivates the dynamic labels.

3. Complementary information

Info: general information about the specimen, as well as a list of the dynamic labels
Drawing: schematic drawing of the specimen
Staining: information about the staining method for this specimen
Knowledge: short texts with basic histologic information, presently deactivated

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