Specimen Nr. 01G

Specimen:

Rat fetus

Staining:

Hopa

Magnification:

250x

Important structures :

1.	Mesenchymal tissue
2.	Osteoblast with (light blue) osteoid border
3.	Osteoclast
4.	Bone trabeculae

Am Rand der dunkler blau gefärbten Knochenmatrix der Knochenbälkchen ist ein schmaler Saum mit hellblau gefärbtem Osteoid zu sehen. Dort, wo Osteoklasten dem Knochenbälkchen aufliegen, fehlt dieser Osteoidsaum. Die Knochenmatrix erscheint wie angenagt.

Legende:

	Mesenchymal tissue
	Osteoblast with (light blue) osteoid border
	Osteoclast
	Bone trabeculae

HOPA

Structures	Colour
Nuclei	brown
Cytoplasm	grey-blue
Connective-tissue fibres - Collagen - Reticular - Elastic	blue blue -
Myocytes	grey-blue to grey-green
Erythrocytes	yellow-orange

Dyes:

HOPA = Hemalaun, Orange G, Phosphormolybdenic acid, Aniline blue

Cell nuclei are stained with hemalaun or Weigerts iron-hematoxylin and then counterstained with a dye mixture of orange-phosphormolybden with aniline blue.

Osteoklasten sind kernreiche Riesenzellen und sollen sich von Blutmonozyten ableiten. Sie resorbieren aktiv und selektiv den Knochen. Die so entstehenden Resorptionsbuchten werden als Howship - Lakunen bezeichnet.

Magnification:

40x

Magnification:

100x

Magnification:

250x

Magnification:

100x

Magnification:

250x

Osteoblast with (light blue) osteoid border

HistoNet2000 - Help

1. Organization of the screen surface

Right side: histologic specimen
Left side: information about the specimen (above) and general program functions (below)

2.Histologic specimen

Pull the mouse across the histologic specimen for training purposes. A small square with exclamation marks (dynamic labels) will appear where there is an important structure. You should then decide what structure this could be. To check your result, simply click the appropriate square, and the correct label will appear. The option “marked” allows you to see all labels for all structures simultaneously. These can be removed by clicking “unmarked”. This reactivates the dynamic labels.

3. Complementary information

Info: general information about the specimen, as well as a list of the dynamic labels
Drawing: schematic drawing of the specimen
Staining: information about the staining method for this specimen
Knowledge: short texts with basic histologic information, presently deactivated

4. General Program Functions

Home: returns you to the “start” page
Tutor: how to contact the HistoNet Team
Help: Instructions for Use appear
Exit: closes down the HistoNet program
Boxes: goes back to the other specimen of a topic
VM: provides virtual microscopy

We hope you will enjoy working with HistoNet2000 and learn a lot from it!