Specimen Nr. 01C

Specimen:

Myenteric neural plexus, Jejunum (Human being)

Staining:

Hopa

Magnification:

44x

Important structures :

1.	Cross section of circular layer of muscular layer
2.	Longitudinal layer of muscular layer (longitudinally sliced)
3.	Perineurium
4.	Nerve cell with sliced nucleus
5.	Nerve cell without sliced nucleus
6.	Nuclei of glial cells

Das Perineurium umgibt das Ganglion. Es besteht neben Kollagenfasern (hier blau gefärbt) aus mesenchymalen Zellen, die untereinander durch tight junctions verbunden sind. Im Ganglion erkennt man zwei Zellarten: die größeren Nervenzellen und die deutlich kleineren Gliazellen.

Legende:

	Cross section of circular layer of muscular layer
	Longitudinal layer of muscular layer (longitudinally sliced)
	Perineurium
	Nerve cell with sliced nucleus
	Nerve cell without sliced nucleus
	Nuclei of glial cells

Ganglion[bu]

1.	Ganglion cell (neuron)
2.	Satellite cells
3.	Nucleus of Schwann cell
4.	Nerve fibre
5.	Lipofuscin (age pigment)
6.	Capillary
7.	Endoneurium
8.	Axon hillock of a ganglion cell
9.	Slice of ganglion cell (neuron)

HOPA

Structures	Colour
Nuclei	brown
Cytoplasm	grey-blue
Connective-tissue fibres - Collagen - Reticular - Elastic	blue blue -
Myocytes	grey-blue to grey-green
Erythrocytes	yellow-orange

Dyes:

HOPA = Hemalaun, Orange G, Phosphormolybdenic acid, Aniline blue

Cell nuclei are stained with hemalaun or Weigerts iron-hematoxylin and then counterstained with a dye mixture of orange-phosphormolybden with aniline blue.

Das Perineurium schirmt den Extrazellulärraum des peripheren Nervengewebes vom Milieu des übrigen Körpers ab (Blut-Nerven-Schranke).

Magnification:

11x

Magnification:

28x

Magnification:

44x

Magnification:

102x

Magnification:

176x

Cross section of circular layer of muscular layer

Longitudinal layer of muscular layer (longitudinally sliced)

Nerve cell with sliced nucleus

Nerve cell without sliced nucleus

Nuclei of glial cells

HistoNet2000 - Help

1. Organization of the screen surface

Right side: histologic specimen
Left side: information about the specimen (above) and general program functions (below)

2.Histologic specimen

Pull the mouse across the histologic specimen for training purposes. A small square with exclamation marks (dynamic labels) will appear where there is an important structure. You should then decide what structure this could be. To check your result, simply click the appropriate square, and the correct label will appear. The option “marked” allows you to see all labels for all structures simultaneously. These can be removed by clicking “unmarked”. This reactivates the dynamic labels.

3. Complementary information

Info: general information about the specimen, as well as a list of the dynamic labels
Drawing: schematic drawing of the specimen
Staining: information about the staining method for this specimen
Knowledge: short texts with basic histologic information, presently deactivated

4. General Program Functions

Home: returns you to the “start” page
Tutor: how to contact the HistoNet Team
Help: Instructions for Use appear
Exit: closes down the HistoNet program
Boxes: goes back to the other specimen of a topic
VM: provides virtual microscopy

We hope you will enjoy working with HistoNet2000 and learn a lot from it!