Specimen Nr. 01D

Specimen:

Myenteric neural plexus, Jejunum (Human being)

Staining:

Hopa

Magnification:

102x

Important structures :

1.	Cross section of circular layer of muscular layer
2.	Longitudinal layer of muscular layer (longitudinally sliced)
3.	Nerve cell body (without nucleus)
4.	Nuclei of glial cells
5.	Perineurium
6.	Nucleus of nerve cell with nucleolus

Bei einigen Zellkörpern der Nervenzellen ist der Zellkern nicht getroffen. Die Wahrscheinlichkeit, am Zellkern vorbeizuschneiden, ist um so höher, je größer eine Zelle ist. Die kleinen Zellkerne, deren Zytoplasma man nicht sieht, sind entweder Gliazellen oder Fibrozyten. Die quergeschnittenen glatten Muskelzellen sind von einem blau gefärbten Ring aus retikulärem Bindegewebe, dem Endomysium, umgeben. In vielen Muskelzellen ist der Zellkern nicht getroffen.

Legende:

	Cross section of circular layer of muscular layer
	Longitudinal layer of muscular layer (longitudinally sliced)
	Nerve cell body (without nucleus)
	Nuclei of glial cells
	Perineurium
	Nucleus of nerve cell with nucleolus

Ganglion[bu]

1.	Ganglion cell (neuron)
2.	Satellite cells
3.	Nucleus of Schwann cell
4.	Nerve fibre
5.	Lipofuscin (age pigment)
6.	Capillary
7.	Endoneurium
8.	Axon hillock of a ganglion cell
9.	Slice of ganglion cell (neuron)

HOPA

Structures	Colour
Nuclei	brown
Cytoplasm	grey-blue
Connective-tissue fibres - Collagen - Reticular - Elastic	blue blue -
Myocytes	grey-blue to grey-green
Erythrocytes	yellow-orange

Dyes:

HOPA = Hemalaun, Orange G, Phosphormolybdenic acid, Aniline blue

Cell nuclei are stained with hemalaun or Weigerts iron-hematoxylin and then counterstained with a dye mixture of orange-phosphormolybden with aniline blue.

Die lichtmikroskopische Vergrösserung reicht nicht aus, um die Gliazellen näher zu charakterisieren. Im Prinzip sind es entweder Schwann’sche Zellen, die die Axone umgeben oder Satellitenzellen (Mantelzellen), die die Nervenzellkörper umgeben. Letztere sind aber nicht so regelmässig angeordnet wie in einem Spinalganglion.

Magnification:

11x

Magnification:

28x

Magnification:

44x

Magnification:

102x

Magnification:

176x

Cross section of circular layer of muscular layer

Longitudinal layer of muscular layer (longitudinally sliced)

Nerve cell body (without nucleus)

Nuclei of glial cells

Nucleus of nerve cell with nucleolus

HistoNet2000 - Help

1. Organization of the screen surface

Right side: histologic specimen
Left side: information about the specimen (above) and general program functions (below)

2.Histologic specimen

Pull the mouse across the histologic specimen for training purposes. A small square with exclamation marks (dynamic labels) will appear where there is an important structure. You should then decide what structure this could be. To check your result, simply click the appropriate square, and the correct label will appear. The option “marked” allows you to see all labels for all structures simultaneously. These can be removed by clicking “unmarked”. This reactivates the dynamic labels.

3. Complementary information

Info: general information about the specimen, as well as a list of the dynamic labels
Drawing: schematic drawing of the specimen
Staining: information about the staining method for this specimen
Knowledge: short texts with basic histologic information, presently deactivated

4. General Program Functions

Home: returns you to the “start” page
Tutor: how to contact the HistoNet Team
Help: Instructions for Use appear
Exit: closes down the HistoNet program
Boxes: goes back to the other specimen of a topic
VM: provides virtual microscopy

We hope you will enjoy working with HistoNet2000 and learn a lot from it!