Specimen Nr. 01E

Specimen:

Myenteric neural plexus, Jejunum (Human being)

Staining:

Hopa

Magnification:

176x

Important structures :

1.Nerve cell body with sliced nucleus and distinct nucleolus
2.Plexus of axons and dendrites (Neuropil)
3.Perineurium
4.Nuclei of glial cells
5.Nerve cell without sliced nucleus
6.Nucleus of smooth muscle cell
Einen Nervenzellkörper erkennt man u.a. an seiner Grösse (in Relation zu den anderen Zellen in der Umgebung), am chromatin-armen Zellkern und am deutlichen Nucleolus (sofern im Schnitt getroffen). Axone, Dendriten und Synapsen sind ohne Elektronenmikroskop nicht zu differenzieren.

Legende:

Nerve cell body with sliced nucleus and distinct nucleolus
Plexus of axons and dendrites (Neuropil)
Perineurium
Nuclei of glial cells
Nerve cell without sliced nucleus
Nucleus of smooth muscle cell

Ganglion[bu]

1. Ganglion cell (neuron)
2. Satellite cells
3. Nucleus of Schwann cell
4. Nerve fibre
5. Lipofuscin (age pigment)
6. Capillary
7. Endoneurium
8. Axon hillock of a ganglion cell
9. Slice of ganglion cell (neuron)

HOPA

Structures Colour
Nuclei brown
Cytoplasm grey-blue
Connective-tissue fibres
- Collagen
- Reticular
- Elastic

blue
blue
-
Myocytes grey-blue to grey-green
Erythrocytes yellow-orange

Dyes:

HOPA = Hemalaun, Orange G, Phosphormolybdenic acid, Aniline blue

Cell nuclei are stained with hemalaun or Weigerts iron-hematoxylin and then counterstained with a dye mixture of orange-phosphormolybden with aniline blue.

Das Neuropil des Plexus myentericus enthält entweder efferente Axone, die von den hier sichtbaren Zellkörpern ausgehen, oder afferente Axone, die aus benachbarten Knotenpunkten kommen. Ausserdem kommen die Endigungen sympathischer und parasympathischer Axone in Frage, die über Synapsen mit den enterischen Neuronen in Verbindung stehen. (Überlege, wo die Zellkörper liegen, von denen diese Axone ausgehen!) Im Hinblick auf ihre Überträgerstoffe sind die Neurone des enterischen Nervensystems sehr heterogen. Es kommen verschiedene Neurotransmitter und Neuropeptide vor.
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Magnification:

11x

Magnification:

28x

Magnification:

44x

Magnification:

102x

Magnification:

176x

Magnifications
Nerve cell body with sliced nucleus and distinct nucleolus
Plexus of axons and dendrites (Neuropil)
Perineurium
Nuclei of glial cells
Nerve cell without sliced nucleus
Nucleus of smooth muscle cell

HistoNet2000 - Help

1. Organization of the screen surface

Right side: histologic specimen
Left side: information about the specimen (above) and general program functions (below)

2.Histologic specimen

Pull the mouse across the histologic specimen for training purposes. A small square with exclamation marks (dynamic labels) will appear where there is an important structure. You should then decide what structure this could be. To check your result, simply click the appropriate square, and the correct label will appear. The option “marked” allows you to see all labels for all structures simultaneously. These can be removed by clicking “unmarked”. This reactivates the dynamic labels.

3. Complementary information

Info: general information about the specimen, as well as a list of the dynamic labels
Drawing: schematic drawing of the specimen
Staining: information about the staining method for this specimen
Knowledge: short texts with basic histologic information, presently deactivated

4. General Program Functions

Home: returns you to the “start” page
Tutor: how to contact the HistoNet Team
Help: Instructions for Use appear
Exit: closes down the HistoNet program
Boxes: goes back to the other specimen of a topic
VM: provides virtual microscopy

We hope you will enjoy working with HistoNet2000 and learn a lot from it!

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